The painting depicts the Virgin Mary with the Child Jesus blessing the Infant Saint John the Baptist, recognizable by the small lamb beside him and the characteristic scroll. Saint Joseph, in the guise of an elderly patriarch, and Saint Anne appear nearby. In the background, two angels holding a scroll complete the composition.
The work is executed on copper plate, a rare and valuable medium that allows for fine application and bright, long-lasting colors. The rich draperies, intense faces, and compositional style reveal a late Mannerist style with Venetian influences.